USDOT number
Issued by the Federal Motor Carrier Safety Administration (FMCSA), the USDOT number identifies companies for safety monitoring.
MC number
The MC (motor carrier) number reflects operating authority for for-hire interstate transportation. Brokers hold broker authority under an MC number.
Looking them up
Anyone can look up public records on the FMCSA SAFER system (safer.fmcsa.dot.gov) and the Licensing and Insurance site (li-public.fmcsa.dot.gov).